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Image Search Results
Journal: Scientific Reports
Article Title: Spatial geometry of stem cell proliferation in the adult hippocampus
doi: 10.1038/s41598-018-21078-6
Figure Lengend Snippet: The distribution of distances between the nearest dividing cells is not different from random. ( A ) Actual positions of all RGLs (grey dots) and BrdU + RGLs (black dots) in a single section from 2.5 month old mouse. This section contained 11 BrdU + RGLs. ( B ) Positions of resampled sham BrdU + RGLs (blue dots) within the same actual positions of all RGLs. For the resampling procedure in this section, we randomly selected N = 11 RGL cells designated as sham labeled cells. Scale bar: 100 μm. ( C , D ) The distributions of nearest neighbor (NN) distances between the centers of actual BrdU + and resampled sham BrdU + RGLs. p > 0.4 calculated with the Kolmogorov-Smirnov test (see Methods). Calculated distances from 74 sections of n = 4 animals were pooled for direct measurement of the distribution of BrdU + cells ( c ), and the calculated distances from equal numbers of randomly selected sections from the same animals were pooled to obtain the distribution of resampled or sham BrdU + RGLs ( D ) (see Methods). ( E ) The distribution of NN distances with 5 μm bins. The black line shows the actual distribution (same data as ( C ), <100 um range) and blue line represents the resampled distribution obtained from 1,000 random samplings. The center values and error bars represent the means and SD. p > 0.03 and q > 0.13 for every bin, computed using bootstrap and FDR correction for multiple hypothesis (see Methods). ( F ) The same analysis for all distances (not necessarily nearest) between actual (black) and randomly resampled (blue) RGL cells. Bin size is 20 μm. p > 0.04 , q > 0.12 for every bin.
Article Snippet: We then tested the hypothesis that the distributions are different in each bin by using the
Techniques: Labeling